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Homographically generated light sheets for the microscopy of large specimens

C. T. Russell, E. J. Rees, and C. F. Kaminski, "Homographically generated light sheets for the microscopy of large specimens," Opt. Lett.43, 663-666 (2018)



We compare the performance of linear and nonlinear methods
for aligning the excitation and detection planes throughout
volumes of large specimens in digitally scanned light
sheet microscopy. An effective nonlinear method involves
the registration of four corner extrema of the imaging volume
via a projective transform. We show that this improves the
light collection efficiency of the commonly used three-point
affine registration by an average of 42% over a typical specimen
volume. Accurate illumination/detection registration
methods are now pertinent to biological research in view
of current trends towards imaging large or expanded samples,
at depth, with diffraction limited resolution.