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Fast and simple spectral FLIM for biochemical and medical imaging

M Popleteeva, K T Haas, D Stoppa, L Pancheri, L Gasparini, C F Kaminski, L D Cassidy, A R Venkitaraman and A Esposito, "Fast and simple spectral FLIM for biochemical and medical imaging," Opt. Express 23, 23511-23525 (2015).

pdf | DOI:10.1364/OE.23.023511


Spectrally resolved fluorescence lifetime imaging microscopy (λFLIM) has powerful potential for biochemical and medical imaging applications. However, long acquisition times, low spectral resolution and complexity of λFLIM often narrow its use to specialized laboratories. Therefore, we demonstrate here a simple spectral FLIM based on a solidstate detector array providing in-pixel histrogramming and delivering faster acquisition, larger dynamic range, and higher spectral elements than stateof-the-art λFLIM. We successfully apply this novel microscopy system to biochemical and medical imaging demonstrating that solid-state detectors are a key strategic technology to enable complex assays in biomedical laboratories and the clinic.