skip to primary navigationskip to content

Seminar by Ricardo Henriques (UCL)

When Feb 26, 2019
from 02:00 PM to 03:00 PM
Where Johnson Matthey Lecture Theatre (LT2), Department of Chemical Engineering and Biotechnology, Philippa Fawcett Drive, Cambridge CB3 0AS
Contact Name
Contact Phone +44 (0)1223 761208
Add event to calendar vCal

'Democratising high-quality live-cell super-resolution microscopy enabled by open-source analytics in ImageJ'

Ricardo Henriques (UCL)

In this talk I will present high-performance open-source approaches we have recently developed to enable and enhance optical super-resolution microscopy in most modern microscopes, these are NanoJ-SRRF, NanoJ-SQUIRREL and NanoJ-Fluidics. SRRF (reads as surf) is a new super-resolution method capable of enabling live-cell nanoscopy with illumination intensities orders of magnitude lower than methods such as SMLM or STED. The capacity of SRRF for low-photoxicity, allows unprecedented imaging for long acquisition times at resolution equivalent or better than SIM.  For the second part of the talk, I will introduce SQUIRREL, an analytical approach that provides quantitative assessment of super-resolution image quality, capable of guiding researchers in optimising imaging parameters. By comparing diffraction-limited images and super-resolution equivalents of the same acquisition volume, this approach generates a quality score and quantitative map of super-resolution defects. To illustrate its broad applicability to super-resolution approaches, we demonstrate how we have used SQUIRREL to optimise several image acquisition and analysis pipelines. Finally, I will showcase a novel fluidics approach to automate complex sequences of treatment, labelling and imaging of live and fixed cells at the microscope. The NanoJ-Fluidics system is based on low-cost LEGO hardware controlled by ImageJ-based software and can be directly adapted to any microscope, providing easy-to-implement high-content, multimodal imaging with high reproducibility. We demonstrate its capacity to carry out complex sequences of experiments such as super-resolved live-to-fixed imaging to study actin dynamics; highly-multiplexed STORM and DNA-PAINT acquisitions of multiple targets; and event-driven fixation microscopy to study the role of adhesion contacts in mitosis.

RSS Feed Latest news

The LAG and MNG attend Focus on Microscopy 2019

Apr 28, 2019

Members of the Laser Analytics Group and the Molecular Neuroscience Group attended the Focus on Microscopy Conference in London.

View all news