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Chetan Poudel

Chetan Poudel

Supercontinuum Radiation for the Development of Automated Microscopy Platforms

Laser Analytics Group

Marie-Sklowdowska Curie Early Stage Researcher (SUPUVIR ITN)


Office Phone: +44 (0)1223 762924

Biography:

B.A., Physics (High Honors) - Oberlin College, 2014
Imaging in Diabetes Research Assistant - University of Wisconsin-Madison, 2014-16
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In the Laser Analytics Group, I work on building and maintaining two fluorescence lifetime imaging microscopes incorporating supercontinuum excitation. One of the microscopes is a home-built confocal microscope based TCSPC-FLIM. The other is an 
automated, drug-screening microscopy platform based on time-gated FLIM. My recent work uses FLIM to study neuro-degeneration in live C. elegans worms, to study chromatin condensation states in living cells and to investigate other sensing applications. 

Key Publications

a. Using fluorescence lifetime imaging (FLIM) for metabolic imaging in the field of diabetes and aging: 
Gregg T.A.*, Poudel C.*, Schmidt B.A., Dhillon R.S., Sdao S.M., Truchan N.A., Baar E.L., Fernandez L.A., Denu J.M., Eliceiri K.W., Rogers J.D., Kimple M.E., Lamming D.W., and Merrins M.J. (2016) ’Pancreatic Beta cells from Mice Offset Age-Associated Mitochondrial Deficiency with Reduced KATP Channel Activity.’ Diabetes, 2016 June.
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b. Simultaneous patch-clamp electrophysiology with FRET measurements and calcium imaging:  
Merrins M.J., Poudel C., McKenna J.P., Ha J., Sherman A., Bertram R. and Satin L.S. (2016) ’Phase Analysis of Metabolic Oscillations and Membrane Potential in Pancreatic Islet Beta Cells’, Biophysical Journal, vol.110, Issue 3, pp.691-699. 
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c. Imaging of metabolic health of pancreatic islets:
Cummings N.E., Williams E.M., Kasza I., Konon E.N., Schaid M.D., Schmidt B.A., Poudel C., Sherman D.S., Yu D., Arriola Apelo S.I., Cottrell S.E., Geiger G., Barnes M.E., Wisinski J.A., Fenske R.J., Matkowskyj K.A., Kimple M.E., Alexander C.M., Merrins M.J., Lamming D.W. (2017) 'Restoration of Metabolic Health by decreased consumption of branched-chain amino acids', Journal of Physiologydoi: 10.1113/JP275075.
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d. Separation of magnetic nanoparticles using a custom-built magnet array:

Ijiri, Y., Poudel, C., Williams,P., Moore, L., Orita, T. and Zborowski, M. (2013) 'Inverted Linear Halbach Array for Separation of Magnetic Nanoparticles’, IEEE Transactions on Magnetics, vol. 49, no. 7, pp. 3449-3452.