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Chetan Poudel

Chetan Poudel

Supercontinuum Radiation for the Development of Automated Microscopy Platforms

Laser Analytics Group

Marie-Sklodowska Curie ESR (SUPUVIR ITN)


Office Phone: +44 (0)1223 762924

Biography:

B.A., Physics (High Honors) - Oberlin College, 2014
Imaging in Diabetes Research Assistant - University of Wisconsin-Madison, 2014-16
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In the Laser Analytics Group, I have built and maintain two fluorescence lifetime imaging (FLIM) microscopes incorporating supercontinuum excitation. One of the microscopes is a home-built confocal based TCSPC-FLIM. The other is an 
automated, widefield-based, time-gated FLIM developed for screening applications. My recent work uses FLIM to study neuro-degeneration in live C. elegans worms, to study chromatin condensation states in living cells and to combine FLIM with other imaging modalities like AFM.

Keywords

  • Optical sensing
  • Neurodegeneration
  • Image analysis
  • Microscopy
  • Bioimaging
  • Spectroscopy

Equipment

  • Lifetime and Spectral Imaging

Key Publications

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1. Applications of supercontinuum radiation in bioimaging: 
Poudel C and Kaminski CF. "Supercontinuum radiation in fluorescence microscopy and biomedical imaging applications." Journal of the Optical Society of America B 2019;36(2). DOI  pdf
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2. Using FLIM for metabolic imaging in diabetes and aging:
Gregg TA*, Poudel C*, Schmidt BA, Dhillon RS, Sdao SM, Truchan NA, Baar EL, Fernandez LA, Denu JM, Eliceiri KW, Rogers JD, Kimple ME, Lamming DW, and Merrins MJ. "Pancreatic Beta cells from Mice Offset Age-Associated Mitochondrial Deficiency with Reduced KATP Channel Activity." Diabetes 2016. DOI pdf
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3. Patch-clamp electrophysiology with FRET and calcium imaging:
Merrins MJ, Poudel C, McKenna JP, Ha J, Sherman A, Bertram R and Satin LS. "Phase Analysis of Metabolic Oscillations and Membrane Potential in Pancreatic Islet Beta Cells." Biophysical Journal 2016, vol.110, Issue 3, pp.691-699. DOI
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4. Separation of magnetic nanoparticles using a custom-built magnet array:

Ijiri Y, Poudel C, Williams P, Moore L, Orita T and Zborowski M. "Inverted Linear Halbach Array for Separation of Magnetic Nanoparticles"IEEE Transactions on Magnetics 2013, vol. 49, no. 7, pp. 3449-3452. DOI