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Congratulations to the authors: Yuetong Jia, Edward Ward, Francesca van Tartwijk, Yutong Yuan, Yuqing Feng, and Clemens Kaminski for this method, now published in Methods and Applications in Fluorescence.

Find their work here : https://iopscience.iop.org/article/10.1088/2050-6120/ae008f

In this image, you can see the autofocus method performing stably well across diverse sample types and staining structures.