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Single molecule translation imaging

The SMTI procedure in xenopus embyos. (a) A fusion construct between a protein of interest and the YFP Venus is (b) introduced into retinal ganglion cell. (c) The cells are dissected and cultured in a dish to study the effect of various chemicals, so-called guidance cues, on local translation in axon tips. (d) This is quantified via the SMTI procedure: segmentation, bleaching, and localisation. (e) Changes can be measured upon cue application even during SMTI acquisitions.
Single molecule translation imaging
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Laser Analytics Group members attend ICON 2018!

Mar 07, 2018

8 members of the Laser Analytics, Molecular Neuroscience, and Quantitative Imaging groups attended the 2nd International Conference on Nanoscopy (ICON). Florian Stroehl and Dr. Katharina Scherer presented posters of their most recent research in structured illumination microscopy and expansion microscopy (respectively), and Marcus Fantham presented a talk on his online first person volumetric data viewer.

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