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The application of frequency-domain Fluorescence Lifetime Imaging Microscopy as a quantitative analytical tool for microfluidic devices

Elder, AD; Matthews, SM; Swartling, J; Yunus, K; Frank, JH; Brennan, CM; Fisher, AC; Kaminski, CF, "The application of frequency-domain Fluorescence Lifetime Imaging Microscopy as a quantitative analytical tool for microfluidic devices", OPTICS EXPRESS 14:5456-5467(2006), DOI:10.1364/OE.14.005456, |pdf 


Abstract

We describe the application of wide-field frequency domain Fluorescence Lifetime Imaging Microscopy (FLIM) to imaging in microfluidic devices. FLIM is performed using low cost, intensity modulated Light Emitting Diodes (LEDs) for illumination. The use of lifetime imaging for quantitative analysis within such devices is demonstrated by mapping the molecular diffusion of iodide ions across a microchannel.