Mahou P, Curry N, Pinotsi D, Kaminski-Schierle G S, Kaminski C F, "Stimulated emission depletion microscopy to study amyloid fibril formation," Proc. SPIE 9331, Single Molecule Spectroscopy and Superresolution Imaging VIII, 93310U (March 9, 2015).
DOI: 10.1117/12.2079320 |
Aggregation of misfolded proteins is a characteristic hallmark of many neurodegenerative disorders, such as Parkinson’s, Alzheimer’s and Huntington’s diseases. The ability to observe these aggregation processes and the corresponding structures formed in vitro or in situ is therefore a key requirement to understand the molecular mechanisms of these diseases. We report here on the implementation and application of Stimulated Emission Depletion (STED) microscopy to visualize the formation of amyloid fibrils in vitro.