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Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons

Ströhl F, Lin JQ, Laine RF, Wong HH, Urbančič V, Cagnetta R, Holt CE, Kaminski CF, "Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons"Sci. Rep. (2017), 7, 709.

DOI: 10.1038/s41598-017-00695-7 | pdf | Supp info 1 | Supp info 2 | Supp info 3 | Supp info 4


Abstract

Local mRNA translation occurs in growing axons enabling precise control of the proteome in response to signals. To measure quantitatively the spatiotemporal dynamics of protein synthesis in growth cones, we further developed a technique for single molecule translation imaging (SMTI). We report that Netrin-1 triggers a burst of β-actin synthesis at multiple non-repetitive sites, particularly in the periphery. The response is remarkably rapid starting within 20 seconds of cue application.