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Development of broadband cavity ring-down spectroscopy for biomedical diagnostics of liquid analytes

Kiwanuka SS, Laurila TK, Frank JH, Esposito A, Blomberg von der Geest K, Pancheri L, Stoppa D, Kaminski CF, "Development of broadband cavity ring-down spectroscopy for biomedical diagnostics of liquid analytes" Analytical Chemistry, 84 (13), pp 5489–5493 (2012) DOI:10.1021/ac301108q pdf 


Abstract

We present a spectrometer for sensitive absorption measurements in liquids across broad spectral bandwidths. The spectrometer combines the unique spectral properties of incoherent supercontinuum light sources with the advantages of cavity ring-down spectroscopy, which is a self-calibrating technique. A custom-built avalanche photodiode array is used for detection, permitting the simultaneous measurement of ring-down times for up to 64 different spectral components at nanosecond temporal resolution. The minimum detectable absorption coefficient was measured to be 3.2 × 10−6 cm−1 Hz−1/2 at 527 nm. We show that the spectrometer is capable of recording spectral differences in trace levels of blood before and after hemolysis.