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A label-free, quantitative assay of amyloid fibril growth based on intrinsic fluorescence

Pinotsi D, Buell AK, Dobson CM, Kaminski Schierle GS and Kaminski CF, "A label-free, quantitative assay of amyloid fibril growth based on intrinsic fluorescence", ChemBioChem (2013), 14 (7), 846-850
DOI: 10.1002/cbic.201300103   |  pdf


Abstract

Kinetic assay of seeded growth: The graph shows the variation in intrinsic fluorescence intensity of amyloid fibrils. Fluorescence increases during the seeded aggregation of α-synuclein seeds with α-synuclein monomeric protein (blue curve) but not when α-synuclein seeds are incubated with β-synuclein monomeric protein (black curve), thus showing that no seeded growth occurred in this case.